|
Download
protocol
238kb
pdf? |
|
Abstract for Flexi® Rabbit Reticulocyte Lysate System
The rabbit reticulocyte in vitro translation system is widely used to
identify mRNA species and characterize their products. This eukaryotic system
has been reported to post-translationally modify proteins via phosphorylation,
acetylation and isoprenylation. Signal peptide cleavage and core glycosylation
also can be achieved by the rabbit reticulocyte lysate system with the addition
of Canine Pancreatic Microsomal Membranes. The Flexi® Rabbit
Reticulocyte Lysate System provides greater flexibility of reaction conditions
than standard rabbit reticulocyte lysate systems. As with the standard
Rabbit Reticulocyte Lysate, the Flexi® Rabbit Reticulocyte Lysate is
optimized for translation by the addition of:
• Hemin, to prevent inhibition of initiation factor eIF-2a
• An energy-generating system consisting of pretested
phosphocreatine kinase and phosphocreatine
• Calf liver tRNAs, to balance the accepting tRNA
populations,
thus optimizing codon usage and expanding the range of mRNAs
that can be translated efficiently
• Micrococcal nuclease, to eliminate endogenous mRNA, thus
reducing background translation
|
