Abstract for TNT® Coupled Reticulocyte Lysate
Systems
Part# TB126
Printed in USA. Revised 3/09.
Instructions for Use of Products L4600,
L4610,
L4950,
L5010,
L5020,
L4601 &
L4611.
The TNT® Coupled Reticulocyte Lysate Systems offer
researchers an alternative for eukaryotic in vitro translations: a single tube, coupled
transcription/translation system. The TNT® Lysate
Systems greatly simplify the process and reduce the time required to obtain in vitro
translation results. Standard rabbit reticulocyte lysate translations commonly use RNA
synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require three
separate reactions with several steps between each reaction. The TNT® Systems bypass many of these steps by incorporating transcription directly in the
translation mix.
In most cases, the TNT® Lysate reactions produce
significantly more protein (two- to sixfold) in a 1.5-hour reaction than standard in vitro
rabbit reticulocyte lysate translations using RNA templates. In comparisons of 35S
incorporation, the TNT® Lysate reactions incorporate
significantly more radiolabel than do standard in vitro translation reactions.
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