Download
protocol
372kb

pdf?

Abstract for TNT^® Coupled Reticulocyte Lysate Systems

The TNT^® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic in vitro translations: a single tube, coupled transcription/translation system. The TNT^® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard rabbit reticulocyte lysate translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require three separate reactions with several steps between each reaction. The TNT^® Systems bypass many of these steps by incorporating transcription directly in the translation mix.

In most cases, the TNT^® Lysate reactions produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than standard in vitro rabbit reticulocyte lysate translations using RNA templates. In comparisons of ³⁵S incorporation, the TNT^® Lysate reactions incorporate significantly more radiolabel than do standard in vitro translation reactions.

Protocols
Index

Part# TB126
Printed in USA. Revised 9/05
Instructions for Use of Products L4600, L4610, L4950, L5010, L5020, L4601 & L4611: Request this protocol.

Printed publications disclaimer.