Abstract for HeLaScribe® Nuclear Extract in vitro Transcription System
The most well characterized cell-free system for in vitro transcription of
eukaryotic genes is derived from HeLa cell nuclei. HeLa nuclear extracts can
support accurate transcription initiation by RNA polymerase II and exhibit both
basal and regulated patterns of RNA polymerase transcription. These nuclear
extracts also are a source for a variety of transcription factors, DNA binding
proteins and the enzymatic machinery involved in RNA processing.
The HeLaScribe® Nuclear Extract included in this system is
prepared by a modification of the method of Dignam et al. (Nucl.
Acids Res. 11, 1475). The system includes all of the
necessary components for in vitro transcription as well as a positive control
template (CMV immediate early promoter DNA). |
