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Abstract for Wizard® Plus Minipreps DNA Purification System
The Wizard® Plus Minipreps DNA Purification System, which
eliminates many of the problems associated with standard miniprep procedures, provides a
simple and reliable method for rapidly isolating plasmid DNA. This system can be used to
isolate any plasmid but works most efficiently when the plasmid is <20,000bp. When
using the standard protocol, the entire miniprep process can be completed in 15 minutes or
less, with no organic extractions or ethanol precipitations. Multiple minipreps may be
easily processed at one time with the Vac-Man® (20-sample capacity,
Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory
Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in nuclease-free
water. The purified plasmid can be used directly for automated fluorescent DNA sequencing.
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