Abstract for Transfectam^® Reagent for the Transfection of Eukaryotic Cells

Part# TB116
Printed in USA. Revised 3/09.
Instructions for Use of Products E1231 & E1232.

Transfectam^® Reagent for the Transfection of Eukaryotic Cells is dioctadecylamidoglycyl spermine (DOGS), a synthetic, cationic lipopolyamine molecule. The spermine group is covalently attached through a peptide bond to the lipid moiety. The strong positive charge contributed by the spermine headgroup gives the molecule a high affinity for DNA (10⁵–10⁶M^–1), coating the DNA with a cationic lipid layer, which facilitates binding to the cell membrane.

Transfectam^® Reagent allows efficient transfection of a wide range of eukaryotic cells. Transfectam^® Reagent has been used for both stable and transient transfections, with both established cell lines and primary cell cultures and for in vivo applications.