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Abstract for Primer Extension System—AMV Reverse Transcriptase
The Primer Extension System is used to determine the location
and level of a target 5´end of RNA. The System uses AMV Reverse
Transcriptase (Cat.# M5101, M5108 and M9004) and an end-labeled primer to
reverse transcribe the target RNA into cDNA. The amount of cDNA synthesized is
proportional to the amount of target RNA, and the length of the cDNA product
reflects the distance from the primer to the 5´end of the RNA. Primer extension
products are typically analyzed on denaturing polyacrylamide gels, and the use
of labeled DNA markers allow the size of the primer extension product to be
determined. The Kanamycin Positive Control RNA (Cat.# C1381), provided in the
form of a 1.2kb in vitro transcription product, has a primer extension product
that is 87 bases in length.
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