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Abstract for pGEM®-luc Vector
The pGEM®-luc Vector is a cassette vector designed to be a
source of the luc gene encoding firefly luciferase, which is found in the
pGL2 Vectors. The plasmid is not intended for the expression of luciferase in
eukaryotic cells.
The pGEM®-luc Vector was constructed by positioning the
luciferase gene (luc) in the center of the multiple cloning region of the
pGEM®-11Zf(–) Vector, providing a number of unique restriction
sites at both ends of the gene. Sites that are surrounded by parentheses are not
unique, as additional sites for each also exists in the luciferase gene. To make
use of these non-unique sites, a partial restriction enzyme digest should be
performed. Note also that using HindIII or NsiI to clone the luciferase gene
will include upstream ATG codons, which may reduce the efficiency of expression
in eukaryotes. |
