Abstract for E. coli Competent Cells
Part# TB095
Printed in USA. Revised 3/09.
Instructions for Use of Products
L1001,
L1011,
L1191,
L1201,
L2001
& L2011.
Promega E. coli Competent Cells are prepared according to
a modified procedure of Hanahan. The competent cells can be used for
many standard molecular biology applications. Competent cells of strains
HB101 and JM109 are available for convenient transformation in two
efficiencies: High Efficiency at greater than 108cfu/µg and
Subcloning Efficiency at greater than 107cfu/µg. JM109 cells
are an ideal host for many molecular biology applications. HB101 cells
are useful for cloning in vectors that do not require alpha-complementation
for blue/white screening. The BMH 71-18 mutS strain is suitable for
use in in vitro mutagenesis procedures where a repair (–) strain is required.
BL21(DE3)pLysS cells can be used with protein expression vectors that are
under the control of the T7 promoter, such as pET vectors. This strain is
lysogenic for lambda-DE3, which contains the T7 bacteriophage gene 1,
encoding T7 RNA polymerase under the control of the lacUV5 promoter.
BL21(DE3)pLysS also contains the pLysS plasmid, which carries the gene
encoding T7 lysozyme. T7 lysozyme lowers the background expression level
of target genes under the control of the T7 promoter but does not interfere
with the level of expression achieved following induction with IPTG.
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