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Abstract for RNAgents® Total RNA Isolation System
The purity and integrity of RNA isolated from tissue or cultured cells are
critical for its effective use in applications such as Northern blot analysis,
oligo(dT) selection of poly(A)+ RNA, cDNA library construction, translation in
vitro and reverse transcription PCR (RT-PCR). In recent years, RT-PCR has
emerged as a powerful method to identify and quantitate tissue-specific rare
mRNAs from small amounts of total RNA and mRNA. In addition, RT-PCR allows for
the cloning of cDNA products without the need for constructing and screening
cDNA libraries. As the use of amplification as a research tool has grown, the
need for methods to rapidly isolate high quality RNA from small amounts of
starting material (i.e., tissue and cultured cells) has also increased. The
RNAgents® Total RNA Isolation System is designed to address these
needs.
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