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Abstract for pGEM®-3Zf(+) Vector
The pGEM®-3Zf(+) Vector is a derivative of the pGEM®-3Z
Vector and contains the origin of replication of the filamentous phage f1. The
plasmid serves as a standard cloning vector, as a template for in vitro
transcription and as a template for the production of circular ssDNA.
The pGEM®-3Zf(+) Vector contains SP6 and T7 RNA polymerase
promoters flanking a multiple cloning region within the alpha-peptide
coding region of beta-galactosidase. Insertional inactivation of the
alpha-peptide allows recombinant clones to be directly identified by color
screening on indicator plates. For induction of ssDNA, bacterial cells with the
F´ episome (e.g., JM109, XL-1 Blue, DH5α™ containing pGEM®-3Zf(+)
Vector recombinants are infected with an appropriate helper phage. The plasmid then
enters the f1 replication mode, and the resulting ssDNA is exported from the cell
as an encapsidated virus-like particle. The exported ssDNA can be used for
mutagenesis in vitro or can be sequenced using the T7 Promoter Primer
or pUC/M13 Forward Primer.
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