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Abstract for pGEM®-11Zf(+) Vector
The pGEM®-11Zf(+) Vector can be used as a standard cloning vector,
as a template for in vitro transcription and for the production of ssDNA. The
plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning
region within the alpha-peptide coding region of beta-galactosidase. Insertional
inactivation of the alpha-peptide allows recombinant clones to be directly
identified by color screening on indicator plates when using appropriate E.
coli strains (e.g., JM109, DH5alpha®, XL1-Blue). The multiple
cloning region contains unique restriction sites for
SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII.
The sequences of Promega vectors are available online at: www.promega.com/vectors/
and also from the GenBank® database. |
