Abstract for pGEM^®-11Zf(–) Vector

Part# TB074
Printed in USA. Revised 10/06.
Instructions for Use of Product P2421.

The  pGEM^®-11Zf(-) Vector can be used as a standard cloning vector, as a template for in vitro transcription and for the production of ssDNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be directly identified by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109, DH5alpha^®, XL1-Blue). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII.

The sequences of Promega vectors are available online at: www.promega.com/vectors/ and from the GenBank^® database.