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Abstract for pGEM®-11Zf(-) Vector
The pGEM®-11Zf(-) Vector can be used as a standard cloning
vector, as a template for in vitro transcription and for the production of ssDNA.
The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple
cloning region within the alpha-peptide coding region of beta-galactosidase.
Insertional inactivation of the alpha-peptide allows recombinant clones to be
directly identified by color screening on indicator plates when using
appropriate E. coli strains (e.g., JM109, DH5alpha®,
XL1-Blue). The multiple cloning region contains unique restriction sites for
SfiI, SacI,
EcoRI, SalI,
XhoI, BamHI,
ApaI, XbaI,
NotI, SphI,
NsiI and HindIII.
The sequences of Promega vectors are available online at: www.promega.com/vectors/
and from the GenBank® database.
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