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Abstract for pGEM®-13Zf(+) Vector
The pGEM®-13Zf(+) Vector can be used as a standard cloning vector, as a
template for in vitro transcription and for the production of ssDNA. The plasmid contains
T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase.
Insertional inactivation of the α-peptide allows recombinant
clones to be directly identified by color screening on indicator plates when using
appropriate E. coli strains (e.g., JM109, DH5α™,
XL1-Blue). The multiple cloning region contains unique restriction sites for SfiI,
NotI and HindIII.
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