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Abstract for pGEM®-9Zf(–) Vector
The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a
versatile range of cloning strategies, efficient synthesis of RNA in vitro and the
production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase
promoters flanking a multiple cloning region within the α-peptide
coding region of β-galactosidase. Insertional inactivation of
the α-peptide allows recombinant clones to be directly
identified by color screening on indicator plates. The multiple cloning region is unique
and includes restriction sites for NsiI,
SpeI, Hind III,
XbaI, EcoR I, SalI and SacI.
The sequences of Promega vectors are available online at www.promega.com/vectors/ and are
also available from the GenBank® database.
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