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Abstract for pGEM®-7Zf(-) Vector
The pGEM®-7Zf(-) Vector is a derivative of the pGEM®-7Zf(+)
Vector and differs from it by the orientation of the filamentous
phage f1 origin of replication. The plasmid serves as a standard cloning vector, as a template for in vitro
transcription and as a template for the production of circular ssDNA.
The plasmid contains
T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the
alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the
alpha-peptide allows recombinant clones to be directly identified by color screening on
indicator plates. The multiple cloning region is unique and includes restriction sites for
ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. The polylinker contains restriction
enzyme sites that produce 5´ overhangs or blunt ends (sensitive to exonuclease III), and
these enzyme sites are flanked on both sides by blocks of restriction sites that generate
3´ overhangs (resistant to exonuclease III).
The sequences of Promega vectors are available online at: www.promega.com/vectors/
and are also available from the GenBank® database. The GenBank®/EMBL
Accession Number is X65311.
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