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Abstract for pGEM®-5Zf(-) Vector
The pGEM®-5Zf(-) Vector is a derivative of the pGEM®-5Zf(+)
Vector and differs from it by the orientation of the origin of replication of
filamentous phage f1. The plasmid serves as a standard cloning vector, as a
template for in vitro transcription, and can be used for the production of
circular ssDNA. The plasmid contains T7 and SP6 RNA polymerase promoters
flanking a multiple cloning region within the alpha-peptide coding region of
beta-galactosidase. Insertional inactivation of the alpha-peptide allows
recombinant clones to be directly identified by color screening on indicator
plates.
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