Abstract for pGEM^®-5Zf(–) Vector

Part# TB068
Printed in USA. Revised 11/06.
Instructions for Use of Product P2351.

The pGEM^®-5Zf(-) Vector is a derivative of the pGEM^®-5Zf(+) Vector and differs from it by the orientation of the origin of replication of filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription, and can be used for the production of circular ssDNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be directly identified by color screening on indicator plates.