Abstract for pSP64 Poly(A) Vector

Part# TB052
Printed in USA. Revised 12/05.
Instructions for Use of Product P1241.

The Riboprobe^® System Vector pSP64 Poly(A) can be used as a standard cloning vector and can also be used for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector can also be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI, (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3′ "polyA" tail of 30 residues.

The sequences of Promega vectors are available online at www.promega.com/vectors/ and are also available from the GenBank^® database (GenBank^®/EMBL Accession Number X65328).