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Abstract for pSP64 Poly(A) Vector
The Riboprobe® System Vector pSP64 Poly(A) can be used as a standard
cloning vector and can also be used for in vitro transcription from the SP6 promoter. The
pSP64 Poly(A) Vector can also be used to generate poly(A)+ transcripts in vitro. The
vector has a stretch of 30 dA:dT residues inserted between the Sac I and EcoR
I sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoR
I (Hind III, Pst I, Sal I, Acc I, Hinc II, Xba
I, BamH I, Ava I, Sma I or Sac I), linearization of
the recombinant plasmid with EcoR I allows the use of SP6 RNA polymerase in vitro
to prepare RNA copies of the inserted sequences that contain a synthetic 3′
"polyA" tail of 30 residues.
The sequences of Promega vectors are available online at www.promega.com/vectors/ and
are also available from the GenBank® database (GenBank®/EMBL
Accession Number X65328).
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