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Abstract for Prime-a-Gene® Labeling System
The Prime-a-Gene® Labeling System is based on the method
developed by Feinberg and Vogelstein, in which a mixture of random
hexadeoxyribonucleotides is used to prime DNA synthesis in vitro from any linear
double-stranded DNA template. With this method it is possible to generate probes
of extremely high specific activity (>1 x 109 cpm/µg), even using
DNA fragments cut from agarose gels. Since the input DNA serves as a template
and remains intact during the reaction, minimal amounts of DNA (25ng) can be
labeled to a high specific activity. Typically, greater than 60% of the labeled
deoxyribonucleotide can be incorporated into the Lambda Control DNA using the
labeling reaction described here. Incorporation may vary from 40–80% with
other samples, depending on the template and reaction conditions used. Using a
template greater than 500bp, probes generated with the Prime-a-Gene®
Labeling System are generally 250–300bp in length and are suitable for a
variety of applications.
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