Abstract for pGEM^®-7Zf(+) Vector

Part# TB048
Printed in USA. Revised 12/06.
Instructions for Use of Product P2251.

The pGEM^®-7Zf(+) Vector is a derivative of the pGEM^®-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription, and can be used for the production of circular ssDNA.

The plasmid contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be directly identified by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generation of unidirectional deletions with the Erase-a-Base^® System. The polylinker contains restriction enzyme sites that produce 5´ overhangs or blunt ends (sensitive to Exonuclease III) flanked on both sides by blocks of restriction sites that generate 3´ overhangs (resistant to Exonuclease III).

The sequences of Promega vectors are available online at www.promega.com/vectors/ and are also available from the GenBank^® database. The GenBank^®/EMBL Accession Number is X65310.