Abstract for pGEM®-3Zf(-) Vector
The pGEM®-3Zf(-) Vector is a derivative of the pGEM®-3Z
Vector and contains the origin of replication of the filamentous phage f1. The
plasmid serves as a standard cloning vector, as a template for in vitro
transcription and as a template for the production of circular ssDNA.
The pGEM®-3Zf(-) Vector contains SP6 and T7 RNA polymerase
promoters flanking a region of multiple cloning sites within the alpha-peptide
coding region of beta-galactosidase. Insertional inactivation of the
alpha-peptide allows recombinant clones to be directly identified by color
screening on indicator plates. For induction of ssDNA, bacterial cells
containing pGEM®-3Zf(-) recombinants are infected with an
appropriate helper phage. The plasmid then enters the f1 replication mode, and
the resulting ssDNA is exported from the cell as an encapsidated virus-like
particle. The exported ssDNA can be used for mutagenesis in vitro or can be
sequenced using the SP6 Promoter Primer (Cat.# Q5011) or pUC/M13 Reverse
Primer (Cat.# Q5401, Q5421).
The sequences of Promega vectors are available online at www.promega.com/vectors/
and are also available from the GenBank® database. The GenBank®/EMBL
Accession Number is X65307. |
