|
Download
protocol
195kb
pdf? |
|
Abstract for pGEM®-4Z Vector
The pGEM®-4Z Vector is intended for use as a standard cloning
vector, as well as for the highly efficient synthesis of RNA in vitro. The
vector carries the lacZ α-peptide and multiple cloning region
arrangement from pUC18. In addition, the vector contains both the SP6 and T7 RNA
polymerase promoters flanking the multiple cloning region. This arrangement
gives rise to a functional α-peptide that is capable of complementing the
product of the lacZΔM15 gene to produce functional β-galactosidase.
Cells with the genotype, lacZΔM15, and also containing the pGEM®-4Z
Vector, will be blue in color when plated on indicator media containing IPTG and
X-Gal. However, when the lacZ α-peptide is disrupted by cloning
into the pGEM®-4Z multiple cloning region, complementation does not
occur and no β-galactosidase activity is produced. Therefore, bacterial
colonies harboring recombinant pGEM®-4Z Vector constructs remain
white.
The sequences of Promega vectors are available online at www.promega.com/vectors/
and are also available from the GenBank® database. The GenBank®/EMBL
Accession Number is X65305. |
