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Abstract for the TNT® Coupled Wheat Germ
Extract Systems
The TNT® Coupled Wheat Germ Extract
Systems offers an alternative for eukaryotic in vitro translation: a
one-tube, coupled transcription/translation system. The TNT®
Extract Systems greatly simplify the process and reduce the time required to
obtain in vitro translation results. Standard wheat germ extract translations
commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase
promoters. This entire process requires separate reactions with several steps
between each reaction. The TNT®
Extracts bypass many of these steps by incorporating transcription directly in
the translation mix. Additionally, the TNT®
Extract reactions often produce significantly more protein (two- to sixfold) in a
1.5-hour reaction than do standard in vitro wheat germ extract translations
using RNA templates.
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