Abstract for Packagene® Lambda DNA Packaging System
The Packagene® Lambda DNA Packaging System is derived from the
unique one-strain host system (Rosenberg). The System is easy to use
because it contains all the components and buffers needed to perform the
packaging reaction in a single tube. Only the addition of substrate DNA is
required. The packaging efficiencies of recombinant DNA using the single-strain
Packagene® System are as efficient as those using a two-strain
system.
The Packagene® Extract is produced from an E. coli C
bacterial host. Libraries constructed using the Packagene® System
are not biased by EcoK restriction of genetic material because E.
coli C lacks the K-12 DNA restriction system. The Positive Control DNA
provided with the system, lambda cI857 Sam7, allows evaluation
of Packagene® System performance without depleting sample DNA.
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