|
Download
protocol
56kb
pdf? |
|
Abstract for EGF Receptor
Epidermal Growth Factor Receptor (EGF Receptor) is a cell-surface
glycoprotein composed of a single polypeptide chain (170kDa) that binds the EGF
peptide (6.1kDa). The EGF Receptor is found in numerous tissues of the body
where the number of molecules can range from 20,000 to 200,000 per cell. The EGF
Receptor consists of an extracellular ligand binding domain, a single
transmembrane region and a cytoplasmic intrinsic tyrosine kinase domain.
Upon ligand binding, the EGF Receptor autophosphorylates, causing the
tyrosine kinase domain of the EGF Receptor to become active. Ligands that bind
the Receptor are EGF, Transforming Growth Factor alpha (TGFalpha ), vaccinia
virus growth factor and amphiregulin. The activated tyrosine kinase domain can
phosphorylate a number of substrates, such as phospho-lipase C-gamma 1,
rasGTPase-activating protein, the proto-oncogene c- erbB-2, lipocortin I, the
serine/threonine kinases MAPK and raf.
|
