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Abstract for pGEM® Express Positive Control Template
The pGEM® Express Positive Control Template may be used to
monitor the performance of the Riboprobe® Systems.
Transcription with SP6 RNA Polymerase produces RNA transcripts of 1,787 bases
and 2,566 bases. The shorter transcript (1,787 bases) is due to the presence of
an SP6 terminator region within the template. The terminator is approximately
50% efficient. Transcription of the template with T7 RNA Polymerase results in
transcripts of 1,065 bases and 2,346 bases. The shorter transcript (1,065 bases)
is due to the presence of a T7 terminator region within the template. The
terminator is approximately 70–80% efficient. Transcription with T3 RNA
Polymerase results in transcripts of 250 bases and 1,525 bases. The shorter
transcript (250 bases) is again due to the presence of the T7 terminator, which
is recognized to some extent by T3 RNA Polymerase.
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