Download
protocol
49kb

pdf?

Abstract for Shrimp Alkaline Phosphatase

Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5′ phosphates from DNA. Unlike Calf Intestinal Alkaline Phosphatase, SAP is completely and irreversibly inactivated by heating at 65°C for 15 minutes. Alkaline phosphatases are said to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5′ termini and may also be used for the dephosphorylation of 5′-phosphorylated ends of DNA for subsequent labeling with [³²P]ATP and T4 Polynucleotide Kinase. SAP is active on 5′ overhangs, 5′ recessed and blunt ends.

Protocols
Index

Part# 9PIM820
Printed in USA. Revised 3/07
Instructions for Use of Product M8201: Request this protocol.

Printed publications disclaimer.