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Abstract for Pfu DNA Polymerase
Pfu DNA Polymerase is a
thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of
nucleotides into duplex DNA in the 5´-->3´ direction in the presence of magnesium. The enzyme
has a molecular weight of approximately 90,000 daltons as estimated
from the predicted amino acid sequence and exhibits 3´-->5´ exonuclease
(proofreading) activity. Pfu DNA Polymerase is
recommended for use in PCR and
primer extension reactions that require high fidelity.
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