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Abstract for RQ1 RNase-Free DNase

RQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and single-stranded DNA endonucleolytically, producing 3´-OH oligonucleotides. RQ1 RNase-Free DNase is used in applications where maintaining the integrity of the RNA is critical. This DNase is used for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR.

In the presence of Mg²⁺, DNase I attacks each strand of DNA independently and the sites of cleavage are distributed in a statistically random fashion (Figure 1). In the presence of Mn²⁺, DNase I cleaves both strands of DNA at approximately the same site to yield fragments that are blunt ended or have protruding termini only one or two nucleotides in length.

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Part# 9PIM610
Printed in USA. Revised 2/05
Instructions for Use of Product M6101: Request this protocol.

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