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Abstract for M-MLV Reverse Transcriptase, RNase H Minus
M-MLV Reverse Transcriptase,
RNase H Minus, is a form of Moloney murine leukemia virus reverse transcriptase
(RNA-dependent DNA polymerase) that has been genetically altered to remove the
associated ribonuclease H activity. Although many researchers are successful in
using M-MLV RT(H+) for analytical and some preparative cDNA applications,
reverse transcriptases lacking RNase H activity provide another option for the
preparation of long cDNAs and libraries containing a high percentage of
full-length cDNA.
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