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Abstract for GoTaq^® Hot Start Green Master Mix

GoTaq^® Hot Start Green Master Mix is a premixed ready-to-use solution containing GoTaq^® Hot Start Polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq^® Hot Start Polymerase contains the high-performance GoTaq^® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets, because it may eliminate or minimize primer-dimer and secondary products. In some cases, hot-start PCR may improve yields.

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Part# 9PIM512
Printed in USA 7/08.
Instructions for Use of Products M5122 & M5123: Request this protocol.

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