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Abstract for AMV Reverse Transcriptase
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization
of DNA using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are
more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme
possesses an intrinsic RNase H activity, but the AMV RT preparation is devoid of other
contaminating DNases and RNases.
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