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Abstract for GoTaq® Hot Start Polymerase
GoTaq® Hot Start
Polymerase contains the high-performance GoTaq® DNA polymerase bound to a proprietary
antibody that blocks polymerase activity. The polymerase activity is
restored during the initial denaturation step when amplification
reactions are heated at 94–95°C for two minutes. This allows for
hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient,
room-temperature reaction setup. Hot-start PCR is advantageous for
some amplification targets, because it may eliminate or minimize
primer-dimer and secondary products. In some cases, hot-start PCR
may improve yields.
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