|
Download
protocol
66kb
pdf? |
|
Abstract for Mung Bean Nuclease
Mung Bean Nuclease belongs to a class of enzymes that demonstrate a preference for
single-stranded nucleic acids, lack sugar specificity and hydrolyze single-stranded
substrates to products with 5′-phosphoryl and 3′-hydroxyl termini. Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA
endonucleolytically to yield 5′-phosphoryl terminated products. While the nuclease
prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations, such as 14 units/μg
of DNA, the enzyme degrades double-stranded DNA preferentially from both ends. Mung Bean
Nuclease has been used for transcript mapping studies, flushing staggered ends and for the
separation of cDNA strands after synthesis with reverse transcriptase and DNA polymerase
I.
|
