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Abstract for M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H
Minus, Point Mutant (M-MLV RT (H–)) is an RNA-dependent DNA polymerase that can be used in
cDNA synthesis with long messenger RNA templates (>5kb). The lack of RNase H activity
is germane to the applications in that this activity can start to degrade templates and
first-strand products when incubation times are long, as they may need to be when making
long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical
and some preparative cDNA applications, reverse transcriptases lacking RNase H activity
provide another option for the preparation of long cDNAs and libraries containing a high
percentage of full-length cDNA.
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