GoTaq^® DNA Polymerase contains GoTaq^® DNA Polymerase, 5X Green GoTaq^® Reaction Buffer and 5X Colorless GoTaq^® Reaction Buffer. The enzyme is a native Taq DNA polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The 5X Green GoTaq^® Reaction Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR. Both buffers contain MgCl₂ at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.