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Abstract for DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a
DNA-dependent DNA polymerase that lacks both the 5´-->3´ and 3´-->5´
exonuclease activities present in intact E. coli DNA polymerase I. It
is used for random primer labeling and in strand displacement amplification.
Klenow Fragment, Exonuclease Minus, will leave a single-base 3´-overhang on a
significant proportion of DNA fragments during fill-in of 5´-overhangs.
Therefore, this enzyme is not recommended for preparation of blunt-ended
fragments for ligation.
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