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Abstract for Tth DNA Polymerase
Tth DNA Polymerase is a thermostable enzyme
that replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth
catalyzes the polymerization of nucleotides into duplex DNA in the 5´-->3´
direction in the presence of magnesium and the polymerization of nucleotides
into DNA using an RNA template in the
5´-->3´ direction in the presence of
manganese. The enzyme has a molecular weight of
94,000 daltons as estimated from the predicted amino acid sequence and exhibits 5´-->3´ exonuclease activity. Tth is
recommended for use in PCR,
RT-PCR, reverse transcription and primer extension
reactions at elevated temperature.
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