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Abstract for DNA Polymerase I
DNA Polymerase I catalyzes template-directed polymerization of nucleotides
into duplex DNA in a 5′ to 3′ direction. DNA Polymerase I also possesses a 3′
to 5′ exonuclease activity or “proofreading” function, which lowers the
error rate during DNA replication, and contains a 5′ to 3′ exonuclease
activity, which enables the enzyme to replace nucleotides in the growing strand
of DNA by nick translation. DNA Polymerase I is capable of catalyzing de novo
synthesis of synthetic homopolymers and provides a convenient method for the
preparation of a variety of defined DNA substrates in the laboratory.
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