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Abstract for Terminal Deoxynucleotidyl Transferase, Recombinant
This enzyme catalyzes the repetitive addition of mononucleotides to the terminal 3′-OH of a DNA initiator,
accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM
Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3′-ends with varying degrees of
efficiency.
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