|

Download
protocol
56kb
pdf? |
|
Abstract for RNA Markers
Promega's RNA Markers are suitable for size estimation of single-stranded RNA from
0.28–6.58kb in glyoxal or formaldehyde agarose gels. The RNA Markers consist of a ladder
of nine RNA transcripts that are synthesized in vitro from specific templates. The sizes
are 281, 623, 955, 1,383, 1,908, 2,604, 3,638, 4,981 and 6,583bp. Two microliters of RNA
prepared in formaldehyde/MOPS Buffer is separated onto a 1% formaldehyde-agarose gel using
MOPS running buffer. After electrophoresis, bands are visualized by ethidium bromide
staining.
|