Abstract for rhSP1 (human)
The SP1 transcriptional control sequence is found in a large
number of viral and cellular promoters, including the HIV long
terminal repeat. The SP1 transcription factor contains contiguous
zinc finger motifs that provide specific DNA binding activity. SP1
factor interacts with other coactivator proteins that can modulate
transcriptional activity. The SP1 recombinant protein is expressed
from a human cDNA clone in Sf9 cells. The sequence of the
oligonucleotide used in the purification is:
5'...GCTCGCCCCGCCCCGATCGAAT...3'
3'...CGAGCGGGGCGGGGCTAGCTTA...5'
Please note that Promega's rhSP1 has not been used in
transcription activation assays. For in vitro transcription protocols
using this type of SP1 preparation, see reference 2 on the pdf
linked above.
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