Abstract for Glo Lysis Buffer
Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid
lysis (within 5 minutes) of cultured mammalian cells without scraping or performing
freeze-thaw cycles. It is fully compatible with Bright-Glo™ and
Steady-Glo®
Assay Reagents for analysis of firefly luciferase expression. The half-life of these
reagents remains the same with or without the use of GLB; it is >5 hours for
Steady-Glo®
and >24 minutes for Bright-Glo™ Reagent.
Firefly luciferase is stable for at least 48 hours at room temperature when
diluted to 2.2 x 10–10M in 1X Glo Lysis Buffer containing 1mg/ml BSA.
Glo Lysis Buffer expands the potential uses of Bright-Glo™ and
Steady-Glo®
Assay Reagents. These products are designed for homogeneous assays; however, GLB enables
them to be used in non-homogeneous formats.
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