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Abstract for TSAP Thermosensitive Alkaline Phosphatase
TSAP Thermosensitive Alkaline Phosphatase
catalyzes the removal of 5′ phosphate groups from DNA, thus
preventing the recircularization and religation of linearized
cloning vector DNA during ligation. It is effective on 3′ overhangs,
5′ overhangs and blunt ends. It is also useful for preparing DNA for
5′ end-labeling by removing existing phosphate groups from the 5′
end.
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