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The siRNA Target Designer program designs oligonucleotides for use with Promega's systems which can be used to produce siRNAs:
It analyzes input DNA or RNA sequence for regions that fit requirements
for design of siRNAs. siRNAs that could target these regions will be displayed along with the sequences of the oligonucleotides needed to produce them with the chosen system, and
the complete input sequence with the siRNA regions highlighted.
In addition, the siRNA sequences presented are linked directly to the NCBI BLAST server allowing easy comparison of
each siRNA sequence to all sequences in Genbank®. If any non-target sequences with only one or two nucleotides
different from the putative siRNA are found, and these sequences
may be present in the cells that will be targeted, there is a chance that non-specific down-regulation effects may occur. In this instance we recommend that a different siRNA sequence
be used.
When you launch the siRNA Target Designer it
will open in a new window 800 pixels wide. When finished with the tool,
please close the window and continue from this page.
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