Abstract: Automated Nucleic Acid Purification from ThinPrep^® PAP Samples for CT/NG Detection by Real-Time PCR and HPV Detection by a Laboratory-Developed Test using Hologic Invader^® ASRs

Part# PS091
Printed in USA.
Samuel Caughron, MD¹, Melissa Melby¹, Rebecca Roberts², and Trista Schagat, PhD^2
1Yellowstone Pathology Institute, Billings, MT
²Promega Corporation, Madison, WI

ThinPrep^® solution is commonly used to preserve cervical swab cells for morphological analysis. We examined automated nucleic acid purification from these samples for molecular assays. DNA isolate by Maxwell^® 16 and analyzed for Chlamydia trachomatis and Neisseria gonorrhea (CT/NG) by real-time PCR showed a high correlation to replicate samples assayed by either Roche COBAS^® AMPLICOR or Gen-Probe APTIMA Combo 2^® Assays in independent laboratories (99% concordance). This same method did not consistently yield DNA of sufficient concentration for Invader^®-based Human Papillomavirus (HPV) detection , so we developed a modified extraction method. This method gave 88% concordance to Digene Hybrid Capture II (HC2). These data show Maxwell^® 16 can be used for automated nucleic acid purification from ThinPrep^® PAP samples for downstream CT/NG and Invader^®-based HPV detection.