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Abstract for A Luminescent Cytotoxicity Assay for Drug Discovery
A. Niles, K. Rashka, D. Lange, B. McNamara, P. Guthmiller, T.
Worzella, M. Scurria*, L. Bernad* and T.L. Riss
Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711
*Promega Biosciences, San Luis Obispo, CA 93401
We have developed a non-lytic, homogeneous, luminescent
assay method that embodies a number of features useful for
cytotoxicity testing in high density multi-well plate formats. This
assay measures a ubiquitous and conserved proteolytic activity
corresponding with loss of cellular membrane integrity. The assay
demonstrates superior performance with respect to sensitivity and
useful linear range of cell numbers when compared with other
commercially available luminescent cytotoxicity assays. The assay
chemistry also demonstrates an improvement in marker half-life and
resulting luminescent signal stability. Lastly, the assay can be
conveniently coupled with a fluorescent measure of viability to
multiplex viability and cytotoxicity assays which offers additional
utility for response normalization and determination of compound
specific mode-of-action. |
