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Abstract for GSH-Glo™ Glutathione Assay: Novel Luminescent Assay to Assess Changes in Glutathione Levels in Cells
N Murphy, J Shultz, F Huang, E Hawkins, S Frackman, W Zhou, T Good, L Bernad, KV Wood
Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711
Glutathione (GSH) a non-protein thiol, is an antioxidant abundantly found in eukaryotic cells. Reactive chemical species and drug-drug interactions often cause a drop in GSH levels either by oxidation or reaction wtih the thiol group. Therefore, measurement of GSH is important in the assessment of toxicological responses.
GSH-Glo™ is a luminescent assay for the detection and quantification of GSH in cultured cells and other biological samples. The GSH-Glo™ assay format is amenable to high throughput screening, making this new method a valuable tool for drug discovery and pharmaceutical applications. |
