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Abstract for Automation of Novel Assays for Cell-Based
Multiplexing
Tracey Worzella, Andrew Niles and Michael Busch*
Promega Corporation, Madison, WI 53711 and *CyBio AG,
Goeschwitzer Strasse 40, 07745, Jena, Germany
We demonstrate the use of automation to perform high-throughput
and ultra high-throughput cell-based multiplexing studies. We
provide protocols and supporting data that demonstrate the
automation synergies of the assays and CyBio CyBi®-Well hardware
described here. The Promega MultiTox-Fluor™ and MultiTox-Glo™ are
novel non-lytic reagents that have been developed to
distinguish between viable and non-viable populations of cells in
the same well. The ratio of live and dead cells can be used to
normalize data in order to reduce error associated with cell
dispensing or clumping. Using independent cell viability and
toxicity readouts within the same well serves as an internal control
and helps identify errors from interferences due to test compounds
or medium
components.
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