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Abstract for High Throughput Bioluminescent Assays for P450s and MAO
James Cali, Mary Sobol, Donping Ma, Troy Good*, Tracey Worzella,
Michael Valley
Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711
*Promega Biosciences, San Luis Obispo, CA
Bioluminescence offers significant advantages for configuring sensitive, simple to perform, homogenous high through-put
enzyme assays. We have developed the bioluminescent P450-Glo™, Cytochrome P450 assays and MAO-GloTM, Monoamine Oxidase Assay for predicting the metabolism-based drug-drug interaction potentials of new chemical entities. P450-Glo™ and
MAO-Glo™ Assays rely on luminogenic probe substrates and the light generating reaction of firefly luciferase to measure the impact of test compounds on several P450s and MAO-A and
MAO-B. P450-Glo™ assays are also used in a cell-based method for measuring P450 gene induction at the P450 enzyme level. This suite of assays is ideal for profiling
compounds against multiple metabolic enzymes with a common luminescent readout. |
