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Abstract for Multiplexing Promega’s Cell-Based Assays Using Beckman Coulter Instrumentation

Tracy Worzella1, Brad Larson1 and David Daniels2
1Promega Corporation, Madison, WI, 2Beckman Coulter, Inc, Fullerton, CA

Here we demonstrate the ability to multiplex select Promega cell-based assays using a standard laboratory liquid handler and plate reader. The Beckman Coulter Biomek® FX liquid handler was used for dispensing cell assay reagents to 96- and 384-well tissue culture plates. The multifunctional DTX 880 Multimode Detector was used to measure luminescent and fluorescent output signals from the same assay plate. The data show the ability to combine assay chemistries with laboratory instrumentation in order to obtain more information from one sample well.


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